Intracellular staining of phosphorylated proteins via flow cytometry
Project Abstract
Detection of phosphorylated proteins using multicolor flow cytometry in primary B cells
Lab Advisor
Naomi Morrissette
Department
Molecular Biology and Biochemistry
Academic Quarter
Fall 2004
Project Topic
Quantitative Analysis of Subpellicular Microtubule Length in Oryzalin-Resistant Toxoplasma lines
Project Abstract
Use of fluorescence microscopy for the construction of a 3D rendition microtubule length in various oryzalin resistant mutant toxplasma gondii lines.
PUBLICATIONS
Oak JS, Deane JA, Kharas MG, Luo J, Lane TE, Cantley LC, Fruman DA, Sjogren's syndrome-like disease in mice with T cells lacking class 1A phosphoinositide-3-kinase.. Proc Natl Acad Sci U S A(2006), .
Hess KL, Donahue AC, Ng KL, Moore TI, Oak J, Fruman DA., Frontline: The p85alpha isoform of phosphoinositide 3-kinase is essential for a subset of B cell receptor-initiated signaling responses.. Eur J Immunol(2004), 34(11):2968-76.
Jean S. Oak, Jonathan A. Deane, Ji Luo, Lewis C. Cantley, and David A. Fruman, A role for phosphoinositide 3-kinase p85beta in B cell development and function. UCI Immunology Fair(2006), .
Jean S. Oak, Jonathan A. Deane, Michael G. Kharas, Linda N. Stiles, Ji Luo*, Lewis C. Cantley*, Thomas E. Lane and David A. Fruman, Deletion of Class IA Phosphoinositide 3-Kinase Leads to Dysregulation of T cell Tolerance and the Development of Primary Sjogren’s syndrome. Gene Regulation and Signaling in the Immune System(2006), .
Deane JA, Kharas MG, Oak JS, Stiles LN, Luo J, Moore TI, Ji H, Rommel C, Cantley LC, Lane TE, Fruman DA, T cell function is partially maintained in the absence of class IA phosphoinositide 3-kinase signaling. Blood(2006), Dec 12; [Epub ahead of print].
